| Autor: |
Dörner T; Department of Internal Medicine, University of Texas Southwestern Medical Center at Dallas 75235-8884, USA., Brezinschek HP, Foster SJ, Brezinschek RI, Farner NL, Lipsky PE |
| Jazyk: |
angličtina |
| Zdroj: |
European journal of immunology [Eur J Immunol] 1998 Feb; Vol. 28 (2), pp. 657-68. |
| DOI: |
10.1002/(SICI)1521-4141(199802)28:02<657::AID-IMMU657>3.0.CO;2-Z |
| Abstrakt: |
Somatic hypermutation and subsequent selection play a significant role in shaping the peripheral B cell repertoire. This repertoire is composed of CD5+ (5%) and CD5- B cells (95%) which are known to traffic through different lymphoid compartments. Previous studies have shown that V(H) gene usage by CD5+ and CD5- B cells is similar, although mutations are more frequent in the latter. However, the effect of mutation and subsequent selection on the expressed V(H) repertoire of CD5+ and CD5- B cells has not been delineated in detail. This study, therefore, analyzed the mutational pattern of individual IgM+/CD5+ and IgM+/CD5- B cells. In both populations, mutations can occur without heavy chain isotype switching. Despite the differences in mutational frequency, the patterns of mutation and subsequent selection were comparable in CD5+ and CD5- B cells. These results imply that although mutations are more frequent in CD5- B cells, the overall mechanisms governing somatic hypermutation and subsequent positive and negative selection are similar in CD5+ and CD5- B cells. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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