| Autor: |
van Zeijl CM; TNO Nutrition and Food Research Institute, Department of Molecular Genetics and Gene Technology, Zeist, The Netherlands., van de Kamp EH, Punt PJ, Selten GC, Hauer B, van Gorcom RF, van den Hondel CA |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of biotechnology [J Biotechnol] 1997 Jan 03; Vol. 59 (3), pp. 221-4. |
| DOI: |
10.1016/s0168-1656(97)00170-3 |
| Abstrakt: |
A method was developed to perform PCR directly on mycelial pellets or colonies treated with NOVOzym 234. The method allows rapid screening of large numbers of transformants of both sporulating and non-sporulating fungi for the presence of (co)transforming plasmid copies or for specific genetic modifications such as gene disruption and site specific integration. PCR fragments of at least 3.2 kb can be obtained. Using this method the identification of specific disruption mutants from Aspergillus niger and Beauveria bassiana was carried out. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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