A rapid RT-PCR based method for the detection of BCR-ABL translocation.
| Autor: | Sidorova JYu; Institute of Haematology and Transfusiology, St Petersburg, Russia., Saltykova LB, Lyschov AA, Zaritskey AYu, Abdulkadyrov KM, Blinov MN |
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| Jazyk: | angličtina |
| Zdroj: | Molecular pathology : MP [Mol Pathol] 1997 Oct; Vol. 50 (5), pp. 266-8. |
| DOI: | 10.1136/mp.50.5.266 |
| Abstrakt: | Aims: To optimise a one step reverse transcriptase polymerase chain reaction (RT-PCR) protocol for BCR-ABL chimaera detection. Methods: Compared with published RT-PCR procedures, this novel approach has at least two advantages. First, the same enzyme is used for both reverse transcription and PCR. Second, amplification of the target (BCR-ABL chimaera) and control gene (ABL) is performed simultaneously in the same tube. Results: On testing 40 chronic myelogenous leukaemia patients and 10 healthy donors there was a specificity for the newly developed technique. In addition, dilution experiments demonstrated that the protocol was highly sensitive. Conclusions: The suggested one step PCR strategy is a simple and reliable way to reveal BCR-ABL chimaeras. |
| Databáze: | MEDLINE |
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