| Autor: |
Wagner E; Lehrstuhl für Mikrobielle Genetik, Universität Tübingen, 72076 Tübingen, Germany., Doskar J; Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University, 61137 Brno, Czech Republic., Götz F; Lehrstuhl für Mikrobielle Genetik, Universität Tübingen, 72076 Tübingen, Germany. |
| Jazyk: |
angličtina |
| Zdroj: |
Microbiology (Reading, England) [Microbiology (Reading)] 1998 Feb; Vol. 144 ( Pt 2), pp. 509-517. |
| DOI: |
10.1099/00221287-144-2-509 |
| Abstrakt: |
A genome map of Staphylococcus carnosus TM300, an important micro-organism in the food industry and long used as a starter culture, was constructed by pulsed-field gel electrophoresis of DNA fragments obtained after digestion with NotI, SfiI and ApaI. The size of the chromosome was estimated to be 2590 kb. The fragments were assembled into a physical map using a combination of complementary methods including multiple and partial digests of genomic DNA, hybridization with homologous gene probes, and cross-Southern hybridization. Fifteen genes or gene clusters were positioned on the physical map by Southern hybridization analysis. The map provides a basis for further analysis of the S. carnosus chromosome. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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