Oligonucleotide ligation assay for detecting mutations in the human immunodeficiency virus type 1 pol gene that are associated with resistance to zidovudine, didanosine, and lamivudine.

Autor: Edelstein RE; Department of Pediatrics, University of Washington, Seattle, USA., Nickerson DA, Tobe VO, Manns-Arcuino LA, Frenkel LM
Jazyk: angličtina
Zdroj: Journal of clinical microbiology [J Clin Microbiol] 1998 Feb; Vol. 36 (2), pp. 569-72.
DOI: 10.1128/JCM.36.2.569-572.1998
Abstrakt: This report describes the detection of mutations in the pol gene of human immunodeficiency virus type 1 associated with resistance to zidovudine, didanosine, and lamivudine by genotyping by an oligonucleotide ligation assay specific codons in the pol gene amplified by PCR. Our studies demonstrate the sensitivity, simplicity, and specificity of this genotyping system.
Databáze: MEDLINE