| Autor: |
Knapp LA; Wisconsin Regional Primate Research Center, University of Wisconsin, Madison 53715, USA., Lehmann E, Piekarczyk MS, Urvater JA, Watkins DI |
| Jazyk: |
angličtina |
| Zdroj: |
Tissue antigens [Tissue Antigens] 1997 Dec; Vol. 50 (6), pp. 657-61. |
| DOI: |
10.1111/j.1399-0039.1997.tb02927.x |
| Abstrakt: |
SIV infection of rhesus macaques is an excellent model for HIV infection of humans. Unfortunately, it is has been difficult to identify macaques expressing particular MHC class I alleles. Here we describe the use of PCR-SSP for Mamu-A*01 typing of rhesus macaques. The Mamu-A*01 allele was amplified from genomic DNA using Mamu-A*01-specific primers and positive PCR products were directly sequenced. Our technique identified 15 Mamu-A*01-positive animals of 68 tested. We validated our molecular analysis by showing that lymphocytes from 8 Mamu-A*01-positive animals expressed Mamu-A*01 as determined by immunoprecipitation and 1-D IEF. The technical simplicity and accuracy of this typing method should facilitate selection of Mamu-A*01-positive rhesus macaques for AIDS virus pathogenesis and vaccine studies. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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