Functional and phenotypic differences of monocyte-derived dendritic cells from allergic and nonallergic patients.

Autor: van den Heuvel MM; Department of Cell Biology and Immunology, Faculty of Medicine, Vrije Universiteit, Amsterdam, The Netherlands., Vanhee DD, Postmus PE, Hoefsmit EC, Beelen RH
Jazyk: angličtina
Zdroj: The Journal of allergy and clinical immunology [J Allergy Clin Immunol] 1998 Jan; Vol. 101 (1 Pt 1), pp. 90-5.
DOI: 10.1016/S0091-6749(98)70198-8
Abstrakt: Background: Several studies suggest a role for dendritic cell in the pathogenesis of allergic disease.
Objective: The purpose of this study was to compare function and phenotype of monocyte-derived dendritic cells (MoDC) from allergic asthmatic patients and healthy control subjects.
Methods: MoDCs were developed by incubating adherent monocytes for 5 days with IL-4 and granulocyte-macrophage colony-stimulating factor. Phenotype was assessed with flow cytometry, and the antigen-presenting function was assessed with the allogeneic mixed leukocyte reaction and an autologous specific antigen presentation.
Results: The morphology of the MoDCs was characteristic for immature dendritic cells. MoDCs from allergic asthmatic patients showed phenotypic differences in the expression of HLA-DR, CD11b, and the high-affinity receptor for IgE. A clearly enhanced accessory potential of MoDCs from atopic asthmatic patients in the mixed leukocyte reaction was also shown. Moreover, house dust mite-specific T-cell proliferation was increased.
Conclusion: This study suggests the involvement of dendritic cells in the pathogenesis of atopic asthma by an increased immunostimulatory capacity of MoDCs.
Databáze: MEDLINE
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