A new application of BCG antibody for rapid screening of various tissue microorganisms.

Autor: Kutzner H; Dermatohistologisches Gemeinschaftslabor, Friedrichshafen, Germany., Argenyi ZB, Requena L, Rütten A, Hügel H
Jazyk: angličtina
Zdroj: Journal of the American Academy of Dermatology [J Am Acad Dermatol] 1998 Jan; Vol. 38 (1), pp. 56-60.
DOI: 10.1016/s0190-9622(98)70539-0
Abstrakt: Background: In routine dermatopathology there is growing demand for a simple, fast, cost-effective, and highly sensitive screening tool for the detection of microorganisms.
Objective: Our purpose was to determine whether immunostaining with polyclonal anti-Mycobacterium bovis (BCG), which is known for its interspecies cross-reactivity, is a suitable screening method for many common microorganisms in dermatopathologic specimens.
Methods: A total of 254 formalin-fixed, paraffin-embedded skin specimens of viral, protozoal, fungal, and bacterial infections were stained with appropriate histochemical stains and with anti-BCG.
Results: Anti-BCG labeled bacteria and fungi with high sensitivity and minimal background staining, but did not react with spirochetes, viruses, or protozoa (Leishmania). The quality and sensitivity of anti-BCG staining were superior to conventional histochemical stains.
Conclusion: Because of its cross-reactivity with many bacteria and fungi as well as its high sensitivity and minimal background staining, the anti-BCG immunostain is a promising screening tool for the detection of the most common bacterial and fungal microorganisms in paraffin-embedded skin specimens.
Databáze: MEDLINE
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