| Autor: |
Köhler H; Federal Institute for Health Protection of Consumers and Veterinary Medicine, Jena, Germany., Lemser B, Müller G, Saalmüller A |
| Jazyk: |
angličtina |
| Zdroj: |
Veterinary immunology and immunopathology [Vet Immunol Immunopathol] 1997 Sep 19; Vol. 58 (3-4), pp. 277-86. |
| DOI: |
10.1016/s0165-2427(97)00023-8 |
| Abstrakt: |
The phenotypic composition of bronchoalveolar lymphocytes (BALL) of healthy untreated pigs and of pigs immunized via aerosol with a live Pasteurella multocida mutant was measured cytofluorometrically in comparison to blood lymphocytes (PBL). Cells were analyzed using monoclonal antibodies against porcine IgM, IgA, IgG and SWC3 in single-colour fluorescence and the following antibody combinations for two-colour fluorescence: CD2/SWC1, SWC1/CD25 and CD4/CD8. In nonimmunized pigs, differences between lymphocyte subsets derived from blood and lung were obvious. The numbers of CD2-SWC1+ T-cells, CD25+ activated lymphocytes and IgG+ cells were significantly higher in the bronchoalveolar compartment. Aerosol immunization with P. multocida caused only insignificant changes in the phenotypic composition of PBL whereas the composition of lung-derived lymphocytes changed markedly. The percentage of CD2-SWC1+ lymphocytes and of CD25+ cells coexpressing the SWC1 antigen in BALL increased up to 10 days after aerosol administration, whereas the percentage of all Ig+ cells decreased. These data reflect an early cellular reaction to an aerogenic immunization with a P. multocida aerosol, characterized by an increase in CD2+ T-cells and a T-cell activation. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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