| Autor: |
Paillard L; CNRS UPR 41, Université de Rennes 1, Campus de Beaulieu, 35042 Rennes cedex, France., Omilli F, Legagneux V, Bassez T, Maniey D, Osborne HB |
| Jazyk: |
angličtina |
| Zdroj: |
The EMBO journal [EMBO J] 1998 Jan 02; Vol. 17 (1), pp. 278-87. |
| DOI: |
10.1093/emboj/17.1.278 |
| Abstrakt: |
During Xenopus early development, gene expression is regulated mainly at the translational level by the length of the poly(A) tail of mRNAs. The Eg family and c-mos maternal mRNAs are deadenylated rapidly and translationally repressed after fertilization. Here, we characterize a short sequence element (EDEN) responsible for the rapid deadenylation of Eg5 mRNA. Determining the core EDEN sequence permitted us to localize the c-mos EDEN sequence. The c-mos EDEN confered a rapid deadenylation to a reporter gene. The EDEN-specific RNA-binding protein (EDEN-BP) was purified and a cDNA obtained. EDEN-BP is highly homologous to a human protein possibly involved in myotonic dystrophy. Immunodepleting EDEN-BP from an egg extract totally abolished the EDEN-mediated deadenylation activity, but did not affect the default deadenylation activity. Therefore, EDEN-BP constitutes the first trans-acting factor for which an essential role in the specificity of mRNA deadenylation has been directly demonstrated. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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