| Autor: |
Higuchi T; Department of Paediatrics, Shinshu University School of Medicine, Matsumoto, Japan., Koike K, Sawai N, Mwamtemi HH, Takeuchi K, Shiohara M, Kikuchi T, Yasui K, Ito S, Yamagami O, Sasaki Y, Okumura N, Kato T, Miyazaki H, Ikeda M, Yamada M, Komiyama A |
| Jazyk: |
angličtina |
| Zdroj: |
British journal of haematology [Br J Haematol] 1997 Dec; Vol. 99 (3), pp. 509-16. |
| DOI: |
10.1046/j.1365-2141.1997.4253232.x |
| Abstrakt: |
In a serum-free liquid culture, thrombopoietin (TPO) selectively stimulated the growth of megakaryocytic cells from CD34-positive cord blood cells. Using these cultured cells, we investigated cytokine production by human megakaryocytes. Day 10 megakaryocytes (2 x 10(5)) secreted > 1000 pg/ml of interleukin (IL)-8, in contrast to small amounts of IL-1beta and IL-6. A time-course study showed that the IL-8 production of megakaryocytes occurred at the late phase of the culture period. The megakaryocyte-conditioned medium had the chemotactic potential of polymorphonuclear leucocytes, which was abrogated by the addition of anti-IL-8 antibody, suggesting the secretion of biologically active IL-8. The combination of TPO and IL-1alpha was required for a significant augmentation of the IL-8 secretion. Direct evidence for IL-8 synthesis in megakaryocytes was provided by reverse transcription-polymerase chain reaction on purified CD41b+ cells and by the detection of intracellular IL-8 in CD41b+ cells. These results suggest that TPO stimulates not only the proliferation and differentiation of the progenitors capable of megakaryocytic lineage expression but also IL-8 release by the megakaryocytic cells with the aid of IL-1. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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