| Abstrakt: |
Our task was to isolate autoantibodies to low-density lipoproteins (LDLP) and characterize them. CNBr-Sepharose and LDLP-based immunoadsorbent were prepared. Autoantibodies were isolated from a pool of human sera from donors by affinity chromatography in a column with immunoadsorbent. Analysis of the eluates from the immunoadsorbents by disk electrophoresis in 9% polyacrylamide gel with sodium dodecyl sulfate and beta-mercaptoethanol revealed 5 components: the second component corresponds to heavy immunoglobulin chains and the fourth to light ones. Analysis of eluates in immunophoresis with monospecific antisera precipitating IgG, IgA, and IgM detected all these immunoglobulins. Enzyme immunoassay of immunoglobins in autoantibody preparations showed the following values: 41% of IgG, 7% of IgA, and 52% of IgM. |
