| Autor: |
Kornilova SV, Iasem P, Grigor'ev DN, Kapinos LE, Totova Ia, Blagoĭ IuP |
| Jazyk: |
ruština |
| Zdroj: |
Biofizika [Biofizika] 1997 May-Jun; Vol. 42 (3), pp. 599-606. |
| Abstrakt: |
Using the method of differential scanning calorimetry, the DNA helix-coil transition studied in solutions (10(-3) M Na+, 10(-3) M tris HCl, pH 7.0) containing divalent metal ions (Mn2+ and Ca2+) at relative metal ion concentrations (Me/PDNA) ranging from 0.2 to 20. Dependences of the melting temperature and enthalpy on the ion relative concentration were stated. The fine structure of melting curves in DNA+Me complexes was observed. The resolution degree of this structure was shown to increase in the range of moderate ion concentrations. The data obtained were compared with values determined by UV-spectroscopy. Association constants were defined for Mn2+ and Ca2+ binding to DNA by the ligand theory. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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