| Autor: |
MacDougall J; CNRS-UPR 9073, Institut de Biologie Physico-chimique, Paris, France., Holst-Hansen P, Mortensen KK, Freistroffer DV, Pavlov MY, Ehrenberg M, Buckingham RH |
| Jazyk: |
angličtina |
| Zdroj: |
Biochimie [Biochimie] 1997 May; Vol. 79 (5), pp. 243-6. |
| DOI: |
10.1016/s0300-9084(97)83511-0 |
| Abstrakt: |
Ribosome release factor (RRF) from Escherichia coli was overproduced from an osmo-expression vector. More than 40% of cell protein was RRF after 6 h of induction. A purification scheme is described that produced 50 mg of RRF from an initial culture of 2 L. The recycling time for ribosomes synthesising the tripeptide fMet-Phe-Leu in vitro in the absence of RF3 was reduced from 40 to 15 s by the addition of purified 1.5 microM RRF. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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