| Autor: |
Pironcheva GL; Institute of Molecular Biology, Bulgarian Academy of Science, Sofia, Bulgaria., Miteva KD, Zlatarev ST, Russev GC |
| Jazyk: |
angličtina |
| Zdroj: |
Cytobios [Cytobios] 1996; Vol. 88 (352), pp. 7-10. |
| Abstrakt: |
Run-on transcription experiments with sonicated chromatin from spermatozoa of healthy men revealed the presence of in vitro transcription. The number of actively transcribing RNA polymerase B molecules in sperm chromatin in vitro, isolated from healthy individuals, and the effect of prostaglandin El, were investigated. The results indicate that the number of RNA polymerase B molecules actively engaged in transcription increased one and a half times when 5 units of prostaglandin E1 was added to the reaction mixtures. These results correlate well with the authors' previous findings that the incorporation of labelled uridine triphosphate into pre-mRNA was higher when prostaglandin E1 was added to the reaction mixtures in run-on transcription experiments, and when sperm chromatin from healthy individuals was used as a template. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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