The amino-terminal region of amyloid precursor protein is responsible for neurite outgrowth in rat neocortical explant culture.

Autor: Ohsawa I; Department of Neurochemistry, National Institute of Neuroscience, Kodaira, Tokyo, Japan., Takamura C, Kohsaka S
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1997 Jul 09; Vol. 236 (1), pp. 59-65.
DOI: 10.1006/bbrc.1997.6903
Abstrakt: We have previously shown that secreted forms of beta-amyloid precursor protein (APP(s)s) promote neurite outgrowth in embryonic rat neocortical explant culture. To determine the region of APP(s) responsible for its biological activity, we produced both amino- and carboxyl-terminal regions of APP(s) using a yeast expression system. The purified fragment corresponding to the amino-terminal region (NAPP) enhanced neurite outgrowth of neocortical explants, but the carboxyl-terminal region fragment did not. The neurite-promoting activity of full length APP(s) and NAPP was blocked by the antibody, 22C11, specific for the amino-terminal region, and the 16-mer peptide of epitope for 22C11 also enhanced neurite outgrowth. However, the 17-mer peptide which contains RERMS sequence did not enhance the neurite outgrowth, but promoted the survival of neocortical neurons in dissociated culture. These findings suggested that the amino-terminal region is responsible for the neurite-promoting activity of APP(s)s.
Databáze: MEDLINE
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