| Autor: |
Lakkis FG; Renal Division, Emory University School of Medicine and Veterans Affairs Medical Center, Atlanta, Georgia 30033, USA. fglakki@emory.edu, Cruet EN, Nassar GM, Badr KF, Pascual DW |
| Jazyk: |
angličtina |
| Zdroj: |
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1997 Jun 27; Vol. 235 (3), pp. 529-32. |
| DOI: |
10.1006/bbrc.1997.6832 |
| Abstrakt: |
Using baculoviral and bacterial systems, we expressed biologically active recombinant rat IL-13 and generated neutralizing rat IL-13 antiserum. Recombinant rat IL-13 produced by baculovirus-infected insect cells stimulated proliferation of TF-1 premyeloid cell line and induced expression of 15-lipoxygenase mRNA in human peripheral blood monocytes. Antiserum generated by immunizing a rabbit with recombinant bacterial rat IL-13 specifically inhibited TF-1 proliferation induced by baculoviral rat IL-13 but did not neutralize human IL-13 mitogenic activity. Western blotting with anti-rat IL-13 serum revealed a approximately 12 kD protein band in supernatants of insect cells infected with recombinant baculovirus carrying the rat IL-13 cDNA. The availability of recombinant rat IL-13 and rat IL-13 antibodies should facilitate studying the role of IL-13 in rat models of human inflammatory disorders. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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