| Autor: |
Van de Vosse E; MGC-Department of Human Genetics, Leiden University, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands., Van der Bent P, Heus JJ, Van Ommen GJ, Den Dunnen JT |
| Jazyk: |
angličtina |
| Zdroj: |
Mammalian genome : official journal of the International Mammalian Genome Society [Mamm Genome] 1997 Jul; Vol. 8 (7), pp. 497-501. |
| DOI: |
10.1007/s003359900483 |
| Abstrakt: |
The disease loci for X-linked Retinoschisis (RS), Keratosis follicularis spinulosa decalvans (KFSD), and Coffin-Lowry syndrome (CLS) have been localized to the same, small region in Xp22 on the human X Chromosome (Chr). To generate a high-resolution map of the available contig in this area, we have used the YAC fragmentation vectors pBP108/ADE2 and pBP109/ADE2 and generated fragmented YACs from a 2.5-Mb YAC (y939H7) spanning the mentioned disease gene candidate regions. Forty-seven fragmented YACs were generated and analyzed, ranging in size from 170 kb to over 2400 kb. The resulting YAC fragmentation panel was used to construct a detailed restriction map of the region and has been used to bin clones and markers. As a deletion panel, it will present a valuable resource for further mapping. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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