| Autor: |
Cannizzo SJ; Division of Pulmonary and Critical Care Medicine, New York Hospital-Cornell Medical Center, New York, USA., Frey BM, Raffi S, Moore MA, Eaton D, Suzuki M, Singh R, Mack CA, Crystal RG |
| Jazyk: |
angličtina |
| Zdroj: |
Nature biotechnology [Nat Biotechnol] 1997 Jun; Vol. 15 (6), pp. 570-3. |
| DOI: |
10.1038/nbt0697-570 |
| Abstrakt: |
This study was designed to evaluate the hypothesis that administration of a replication-deficient, recombinant adenovirus vector to the epithelial surface of the respiratory tract can be used to deliver a recombinant protein to the systemic circulation in sufficient quantities to evoke a systemic response appropriate to the recombinant protein. We administered AdCMV.TPO-an adenovirus vector containing an expression cassette coding for the human thrombopoietin (TPO) cDNA-to the respiratory epithelium of immunocompetent Balb/c mice. Over the following week, serum human TPO levels were elevated, platelet levels increased more than sixfold, and megakaryocytosis was evident in bone marrow. This strategy may be a useful approach to the nonparenteral administration of a variety of therapeutic recombinant proteins, such as those relevant to clotting, endocrine function, and bone-marrow function. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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