In situ nitric oxide (NO) measurement by modified electrodes: NO labilized by photolysis of metal nitrosyl complexes.

Autor: Kudo S; Department of Chemistry, University of California, Santa Barbara 93106-9510, USA., Bourassa JL, Boggs SE, Sato Y, Ford PC
Jazyk: angličtina
Zdroj: Analytical biochemistry [Anal Biochem] 1997 May 01; Vol. 247 (2), pp. 193-202.
DOI: 10.1006/abio.1997.2097
Abstrakt: Described are studies directed at refining quantitative analysis of nitric oxide in solution using electrochemical techniques. The fabrication and behavior of several sensors based on modified carbon-based electrodes are reported. This technique has been used to resolve the vexing problem of determining the stoichiometry of the photochemical decomposition of the known antihypertension agent sodium nitroprusside, Na2[Fe(CN)5NO], as well as of two other metal nitrosyl complexes of biological interest, Roussin's black salt, NH4[Fe4S3(NO)7], and Roussin's red salt, Na2[Fe2S2(NO)4], in aqueous solutions. In this manner it was shown that the molar ratios of nitric oxide produced per starting complex photochemically decomposed were 0.95 +/- 0.03, 5.9 +/- 0.2, and 0.50 +/- 0.02 for Na2[Fe(C-N)5NO], NH4[Fe4S3(NO)7], and Na2[Fe2S2(NO)4], respectively.
Databáze: MEDLINE