| Autor: |
Valentich JD; Department of Internal Medicine, University of Texas Medical Branch, Galveston 77555, USA. jdv@metamatrix.com, Popov V, Saada JI, Powell DW |
| Jazyk: |
angličtina |
| Zdroj: |
The American journal of physiology [Am J Physiol] 1997 May; Vol. 272 (5 Pt 1), pp. C1513-24. |
| DOI: |
10.1152/ajpcell.1997.272.5.C1513 |
| Abstrakt: |
Subepithelial myofibroblasts are located at the interface between the epithelium and lamina propria in most mucosal tissues. Their biological functions are largely unknown because a long-term cell culture model for these cells has not been available. In this report, we define the phenotypic properties of a human colonic cell line (18Co) that exhibits most of the known characteristics of intestinal subepithelial myofibroblasts in situ. These characteristics include 1) a cell shape that can be reversibly interconverted between a flattened discoid and stellate morphology, 2) intracellular organelles reminiscent of myofibroblasts and smooth muscle cells in situ, 3) expression of alpha-smooth muscle actin, 4) plasma membrane receptors for endothelins and natriuretic peptides, and 5) regulation of epithelial sensitivity to calcium-dependent secretagogues by paracrine secretion of prostaglandins. 18Co cells provide an exploitable model to begin defining the physiological and pathophysiological functions of intestinal subepithelial myofibroblasts at the molecular level. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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