DNA ploidy and proliferating cell nuclear antigen image analysis of peritoneal and pleural effusions. A possible diagnostic role.
| Autor: | el-Habashi AH; Pathology Department, Cairo University, Egypt., Freeman SM, el-Morsi B, el-Didi M, Martin A, Marrogi AJ |
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| Jazyk: | angličtina |
| Zdroj: | Acta cytologica [Acta Cytol] 1997 May-Jun; Vol. 41 (3), pp. 636-48. |
| DOI: | 10.1159/000332677 |
| Abstrakt: | Objective: To determine the role of DNA and proliferating cell nuclear antigen (PCNA) image analysis (IA) in enhancing the diagnostic sensitivity of conventional cytology (CC). Study Design: The histopathologic and clinical data on 87 consecutive pleural and peritoneal effusions were used to evaluate the accuracy of CC and DNA IA results. Results: CC showed a sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of 65%, 100%, 100% and 62%, respectively. Aneuploidy peaks were seen in 49 cases; 47 of them were true positives. Thirty of 38 diploid cases were true negatives. The sensitivity, specificity, PPV and NPV were 85%, 94%, 96% and 80%, respectively. There were positive correlations between DNA ploidy profile and PCNA proliferative index (PI), (R = .697) and significant differences in PCNA PI between malignant and benign effusions (P < .001). Conclusion: The DNA IA PI by PCNA can be used as a complementary diagnostic tool with CC in cytologically inconclusive cases. |
| Databáze: | MEDLINE |
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