Isolation of pathogen/stress-inducible cDNAs from alfalfa by mRNA differential display.

Autor: Truesdell GM; School of Biological Sciences, University of Nebraska-Lincoln, 68583-0722, USA., Dickman MB
Jazyk: angličtina
Zdroj: Plant molecular biology [Plant Mol Biol] 1997 Mar; Vol. 33 (4), pp. 737-43.
DOI: 10.1023/a:1005728420374
Abstrakt: Differential display of mRNA was used to isolate a full-length (SRG1) and a partial (SRG2) alfalfa cDNA induced during infection with the fungal pathogen Colletotrichum trifolii. The deduced amino acid sequences are similar to each other and resemble plant defense-related proteins and tree pollen allergens. SRG1 is a member of a gene family in alfalfa, which may also include the putative defense-related gene PR10. Unlike many defense-related genes described in similar systems, expression of SRG1-like genes does not correlate with resistance to C. trifolii. We speculate SRG1 is induced in response to plant stress.
Databáze: MEDLINE