Evaluation of the polymerase chain reaction test for detecting chlamydial DNA in adult chlamydial conjunctivitis.
| Autor: | Kowalski RP; Department of Ophthalmology, University of Pittsburgh, PA, USA., Uhrin M, Karenchak LM, Sweet RL, Gordon YJ |
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| Jazyk: | angličtina |
| Zdroj: | Ophthalmology [Ophthalmology] 1995 Jul; Vol. 102 (7), pp. 1016-9. |
| DOI: | 10.1016/s0161-6420(95)30919-0 |
| Abstrakt: | Purpose: A new polymerase chain reaction (PCR) test (Amplicor, Diagnostics, Branchburg, NJ) was evaluated for its ability to detect chlamydial DNA from previously obtained adult conjunctival specimens. Methods: The sensitivity of this PCR test was determined on 42 adult conjunctival specimens that were culture-positive for Chlamydia. The specificity was determined by testing 40 true-negative specimens that included 10 normal conjunctival samples and 20 ocular specimens that were culture-positive for herpes simplex virus or adenovirus. The remaining ten samples consisted of ocular bacterial pathogens in chlamydial transport media. Results: Amplicor was 88% (37/42) sensitive and 100% (40/40) specific. Conclusions: The authors conclude that PCR testing for chlamydial DNA from ocular specimens may be useful, especially when conditions in transport might reduce the yield of positive cultures. |
| Databáze: | MEDLINE |
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