| Autor: |
Malgrange B; Department of Human Physiology and Pathophysiology, University of Liège, Belgium., Rigo JM, Lefebvre PP, Coucke P, Goffin F, Xhauflaire G, Belachew S, Van de Water TR, Moonen G |
| Jazyk: |
angličtina |
| Zdroj: |
Neuroreport [Neuroreport] 1997 Feb 10; Vol. 8 (3), pp. 591-6. |
| DOI: |
10.1097/00001756-199702100-00003 |
| Abstrakt: |
Using dissociated spiral ganglion cell cultures obtained from 3-day-old rat cochlea, we investigated the response of auditory neurones to gamma-aminobutyric acid (GABA) using patch-clamp techniques. In our recording conditions, GABA elicited inward currents in > 95% of the neurones which reversed around 0 mV. Similar inward currents were measured using isoguvacin, a specific agonist of GABAA receptors. GABA-gated currents were reversibly inhibited by the channel blocker picrotoxin and the GABA competitive antagonist bicuculline. These functional GABAA receptors are characterized by an insensitivity to benzodiazepines and a relatively high sensitivity to beta-carbolines and barbiturates. These results show that the GABAA receptor pharmacological properties of spiral ganglion neurones are close to those of cerebellar granule cells. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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