| Autor: |
Boisvert J; Department of Pharmacology, Université de Montréal, Qué., Canada., Caillé G, McGilveray IJ, Qureshi SA |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography. B, Biomedical sciences and applications [J Chromatogr B Biomed Sci Appl] 1997 Mar 07; Vol. 690 (1-2), pp. 189-93. |
| DOI: |
10.1016/s0378-4347(96)00402-1 |
| Abstrakt: |
An HPLC method for the quantification of ketoprofen enantiomers in human plasma is described. Following extraction with a disposable C18 solid-phase extraction column, separation of ketoprofen enantiomers and I.S. (3,4-dimethoxy benzoic acid) was achieved using a chiral column [Chirex 3005; (R)-1-naphthylglycine 3,5-dinitrobenzoic acid] with the mobile phase, 0.02 M ammonium acetate in methanol, set at a flow-rate of 1.2 ml/min. Baseline separation of ketoprofen enantiomers and I.S., free from interferences, was achieved in less than 20 min. The calibration curves (n = 14) were linear over the concentration range of 0.16 to 5.00 micrograms/ml per enantiomer [mean r2 of 0.999 for both enantiomers, root mean square error were 0.015 for R(-) and 0.013 for S(+)]. The inter-day coefficient of variation for duplicate analysis of spiked samples was less than 7% and the accuracy was more than 93% over the over the concentration range of 0.2 to 4.0 micrograms/ml for individual enantiomer using 1 ml of plasma sample. This method has been applied to a pharmacokinetic study from healthy human volunteers following the administration of a ketoprofen extended release product (200 mg). This method is simple, fast and should find wide application in monitoring pharmacokinetic studies of ketoprofen. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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