| Autor: |
Komaromy-Hiller G; Department of Pathology, University of Utah School of Medicine, Salt Lake City 84132, USA., Sundquist PD, Jacobsen LJ, Nuttall KL |
| Jazyk: |
angličtina |
| Zdroj: |
Annals of clinical and laboratory science [Ann Clin Lab Sci] 1997 Mar-Apr; Vol. 27 (2), pp. 163-8. |
| Abstrakt: |
Serum succinate may offer an alternate analyte to lactate for the evaluation of hypoxia. To evaluate the potential uses of succinate, a relatively rapid capillary zone electrophoresis assay was developed for use in the clinical laboratory setting. Employing a simple indirect ultraviolet detection method with commercially available instrumentation, the limit of detection for serum succinate was determined to be 0.1 mumol/L, the upper limit of linearity 100 mumol/L, and the between-run coefficient of variation about 15 percent. Based on specimens from 202 apparently healthy adults, the non-parametric reference interval was 1.0 to 9.2 mumol/L. Preliminary studies in stored blood show succinate increased 2-fold while lactate increased 11-fold, suggesting that succinate may be a clinically useful marker for hypoxia in patients after blood transfusion. This assay provides a practical tool for the investigation of the clinical applications of succinate. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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