| Autor: |
Takagi Y; Faculty of Agriculture, Shinshu University, Minamiminowa, Nagano, Japan., Talbot NC, Rexroad CE Jr, Pursel VG |
| Jazyk: |
angličtina |
| Zdroj: |
Molecular reproduction and development [Mol Reprod Dev] 1997 Apr; Vol. 46 (4), pp. 567-80. |
| DOI: |
10.1002/(SICI)1098-2795(199704)46:4<567::AID-MRD14>3.0.CO;2-T |
| Abstrakt: |
Monoclonal antibodies anti-SSEA-1 and EMA-1, and the lectins DBA and LTA, bound to the surface of large, round cells randomly distributed in the 26-day pig genital ridge. Other antibodies, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81, did not react with any cells in the pig genital ridge. SSEA-1-positive cells displayed pseudopods and appeared to migrate from the dorsal mesentery of the hindgut (18-day) to the primordium of the gonad (day 23) and entered the genital ridge by 26 days. The number of SSEA-1-positive cells associated with the dorsal mesentery and genital ridge markedly increased from the 18-day to the 26-day pig embryo. It was concluded that the SSEA-1-positive cells were primordial germ cells (PGCs). Using these markers and alkaline phosphatase histochemistry, pig PGCs derived from the 26-day genital ridge showed no proliferation when grown in STO co-culture in the presence of human LIF, bFGF and SCF. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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