| Autor: |
Chen B; Department of Crop Protection, Waite Agricultural Research Institute, University of Adelaide, Glen Osmond, South Australia. chenb@raid0.dnet.roche.com, Randles JW, Francki RI |
| Jazyk: |
angličtina |
| Zdroj: |
The Journal of general virology [J Gen Virol] 1995 Apr; Vol. 76 ( Pt 4), pp. 971-3. |
| DOI: |
10.1099/0022-1317-76-4-971 |
| Abstrakt: |
Virus particles were reassembled in vitro from tomato aspermy virus strain V (V-TAV) RNA and a mixture of subunits prepared from V-TAV and 35S-labelled cucumber mosaic virus strain T (T-CMV). Immunodiffusion tests showed that the reassembled particles reacted with polyclonal antisera raised against both V-TAV and T-CMV. Radioactivity was found in the precipitin line formed between the reassembled particles and antiserum raised against T-CMV as well as in the precipitin line formed between the reassembled particles and antiserum raised against V-TAV. This shows that 35S-labelled T-CMV protein subunits were incorporated with V-TAV protein subunits into the same particles. Thus, coat proteins of V-TAV and T-CMV can co-assemble and form mixed-subunit capsids in vitro. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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