| Autor: |
Douglas RS; Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia 19104-6082, USA., Capocasale RJ, Lamb RJ, Nowell PC, Moore JS |
| Jazyk: |
angličtina |
| Zdroj: |
Blood [Blood] 1997 Feb 01; Vol. 89 (3), pp. 941-7. |
| Abstrakt: |
Chronic lymphocytic leukemia (CLL) is the most common leukemia of the western world and is characterized by a slowly progressing accumulation of clonal CD5+ B cells. Our laboratory has investigated the role of transforming growth factor-beta (TGF-beta) and interleukin-4 (IL-4) in the pathogenesis of B-cell expansion in CLL. In vitro addition of TGF-beta did not increase spontaneous apoptosis of B cells from most CLL patients, as determined using the TUNEL method, compared with a twofold increase observed in cultures of normal B cells. There was similar expression of TGF-beta type II receptors on both CLL B cells and normal B cells. In contrast to apoptosis, CLL B-cell proliferation was variably inhibited with addition of TGF-beta. In vitro addition of IL-4, previously reported to promote CLL B-cell survival, dramatically reduced spontaneous apoptosis of CLL B cells compared with normal B cells. CLL B-cell expression of IL-4 receptors was increased compared to normal B cells. Thus, our results show aberrant apoptotic responses of CLL B cells to TGF-beta and IL-4, perhaps contributing to the relative expansion of the neoplastic clone. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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