| Autor: |
Tewksbury DA, Premeau MR, Dumas ML, Frome WL |
| Jazyk: |
angličtina |
| Zdroj: |
Circulation research [Circ Res] 1977 Oct; Vol. 41 (4 Suppl 2), pp. 29-33. |
| DOI: |
10.1161/01.res.41.4.29 |
| Abstrakt: |
A four-step method for the purification of human angiotensinogen has been devised. The four steps are: (1) removal of albumin by affinity chromatography on blue dextran-Sepharose, (2) chromatography on DEAE-Sephadex, (3) chromatography on hydroxylapatite, and (4) chromatography on DEAE-cellulose. This method is capable of producing 8 mg of purified angiotensinogen from 150 ml of plasma with 33% overall recovery of renin-releasable angiotensin I. The angiotensinogen appears homogeneous by immunochemical and ultracentrifugal techniques. The N-terminal amino acids have been determined to be alanine and aspartic acid or asparagine. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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