[Gel-permeating chromatography of interacting multi-component systems. I. Study of protein isomerization by the method of gel-permeation chromatography. II. Method of determining the kinetic constant of isomerization].

Autor: Kurenbin OI, Vilenchik LZ, Gotlib IuIa, Belen'kiĭ BG
Jazyk: ruština
Zdroj: Biofizika [Biofizika] 1977 Jul-Aug; Vol. 22 (4), pp. 589-93.
Abstrakt: A technique is suggested of determining the isomerization constants by means of gel-permeation chromatography. Chromatograms of proteins and expressions for statistical moments of their distribution along the chromatographic column obtained in the authors previous work [1], are used. A necessary condition for optimal setup of the GPCh experiments is found. Fulfilment of this condition provides a maximal accuracy of determination of the isomerization constants.
Databáze: MEDLINE