| Autor: |
Chernov AV, Lepikhov KA, Zheleznaia LA, Matvienko NI |
| Jazyk: |
ruština |
| Zdroj: |
Biokhimiia (Moscow, Russia) [Biokhimiia] 1996 Oct; Vol. 61 (10), pp. 1837-47. |
| Abstrakt: |
Two site-specific endonucleases, BspOVI and BspOVII, were isolated from a thermophilic strain Bacillus species OV. The activities of both enzymes are maximal at 48 degrees C and do not depend on ATP and S-adenosyl-L-methionine. BspOVI recognizes the sequence [sequence: see text] and cleaves it as indicated by arrows. Thus, BspOVI is a IIN-subclass endonuclease isoshizomer of Eam1105I. BspOVI is very stable during storage. The enzyme can be used for direct T/A cloning of PCR products. BspOVII recognizes and cleaves the sequence [sequence: see text]; thus, BspOVII is an isoshizomer of CIaI. The cleavage by BspOVII is blocked by dam methylation of adenine inside the recognition site. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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