| Autor: |
Van Os EC; Department of Medicine, Mayo Clinic/Mayo Foundation, Rochester, MN 59905, USA., McKinney JA, Zins BJ, Mays DC, Schriver ZH, Sandborn WJ, Lipsky JJ |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of chromatography. B, Biomedical applications [J Chromatogr B Biomed Appl] 1996 Apr 26; Vol. 679 (1-2), pp. 147-54. |
| DOI: |
10.1016/0378-4347(96)00007-2 |
| Abstrakt: |
A specific, sensitive, single-step solid-phase extraction and reversed-phase high-performance liquid chromatographic method for the simultaneous determination of plasma 6-mercaptopurine and azathioprine concentrations is reported. Following solid-phase extraction, analytes are separated on a C18 column with mobile phase consisting of 0.8% acetonitrile in 1 mM triethylamine, pH 3.2, run on a gradient system. Quantitation limits were 5 ng/ml and 2 ng/ml for azathioprine and 6-mercaptopurine, respectively. Peak heights correlated linearly to known extracted standards for 6-mercaptopurine and azathioprine (r = 0.999) over a range of 2-200 ng/ml. No chromatographic interferences were detected. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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