Specificity analysis of murine monoclonal antibodies reactive with Tn, sialylated Tn, T, and monosialylated (2-->6) T antigens.
| Autoři: | O'Boyle KP; Montefiore Medical Center/Albert Einstein Cancer Center, Bronx, New York, USA., Markowitz AL, Khorshidi M, Lalezari P, Longenecker BM, Lloyd KO, Welt S, Wright KE |
|---|---|
| Zdroj: | Hybridoma [Hybridoma] 1996 Dec; Vol. 15 (6), pp. 401-8. |
| Způsob vydávání: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
| Jazyk: | English |
| Informace o časopise: | Publisher: Mary Ann Liebert Country of Publication: United States NLM ID: 8202424 Publication Model: Print Cited Medium: Print ISSN: 0272-457X (Print) Linking ISSN: 0272457X NLM ISO Abbreviation: Hybridoma Subsets: MEDLINE |
| Imprint Name(s): | Publication: -2001: Larchmont, NY : Mary Ann Liebert Original Publication: New York : M.A. Liebert, Inc., c1981-c2001. |
| Výrazy ze slovníku MeSH: | Antibodies, Monoclonal/*immunology , Antibody Specificity/*immunology , Antigens, Neoplasm/*immunology , Antigens, Tumor-Associated, Carbohydrate/*immunology , Sialoglycoproteins/*immunology, Animals ; Antibodies, Monoclonal/biosynthesis ; Antibodies, Neoplasm/biosynthesis ; Antigens, Viral, Tumor/immunology ; Enzyme-Linked Immunosorbent Assay ; Humans ; Immunohistochemistry ; Mice |
| Abstrakt: | T, Tn, and sialyated Tn (sTn) are pancarcinoma antigens, and increased expression of these carbohydrate epitopes has been correlated with a poor prognosis in several epithelial malignancies. Ten murine monoclonal antibodies have been generated to these antigens, and compared by ELISA and immunohistochemistry to established mAbs reactive with these antigens. Nine mAbs (3 IgM and 6 IgG) reactive with synthetic T-human serum albumin (T-HSA) were produced after immunizing BALB/c mice with a synthetic T-keyhole limpet hemocyanin glycoconjugate (T-KLH). An additional IgM mAb (145.22) was produced in mice immunized with erythrocytes isolated from a patient with Tn syndrome. Three IgM and six IgG1 mAbs reactive with T-HSA did not react with natural T antigen present on desialyated glycophorin. All three IgM and several IgG1 mAbs, however, did react with LS-174T, a mucinous colon carcinoma cell line, 647V, a human bladder carcinoma cell line, and TA3Ha, a murine mammary carcinoma cell line as well as fresh frozen colon carcinomas. MAb 145.22 reacted with both natural and synthetic sources of sTn and Tn, as well as with LS-174T cells and mucin deposits in 10/11 colon carcinomas on fresh-frozen sections. MAb B72.3 reacted strongly with ovine submaxillary mucin (OSM) and sTn-HSA, while mAb CC49, a second-generation mAb to TAG-72 carcinoma mucin, reacted strongly with OSM, less strongly with desialyated OSM, and only weakly with sTn-HSA, suggesting that the epitope specificity for mAb CC49 is distinct from that of B72.3. |
| Grant Information: | 5 P30CA1330-22 United States CA NCI NIH HHS |
| Substance Nomenclature: | 0 (Antibodies, Monoclonal) 0 (Antibodies, Neoplasm) 0 (Antigens, Neoplasm) 0 (Antigens, Tumor-Associated, Carbohydrate) 0 (Antigens, Viral, Tumor) 0 (Sialoglycoproteins) 0 (Tn antigen) |
| Entry Date(s): | Date Created: 19961201 Date Completed: 19970320 Latest Revision: 20071114 |
| Update Code: | 20240829 |
| DOI: | 10.1089/hyb.1996.15.401 |
| PMID: | 8985750 |
| Autor: | O'Boyle KP; Montefiore Medical Center/Albert Einstein Cancer Center, Bronx, New York, USA., Markowitz AL, Khorshidi M, Lalezari P, Longenecker BM, Lloyd KO, Welt S, Wright KE |
| Jazyk: | angličtina |
| Zdroj: | Hybridoma [Hybridoma] 1996 Dec; Vol. 15 (6), pp. 401-8. |
| DOI: | 10.1089/hyb.1996.15.401 |
| Abstrakt: | T, Tn, and sialyated Tn (sTn) are pancarcinoma antigens, and increased expression of these carbohydrate epitopes has been correlated with a poor prognosis in several epithelial malignancies. Ten murine monoclonal antibodies have been generated to these antigens, and compared by ELISA and immunohistochemistry to established mAbs reactive with these antigens. Nine mAbs (3 IgM and 6 IgG) reactive with synthetic T-human serum albumin (T-HSA) were produced after immunizing BALB/c mice with a synthetic T-keyhole limpet hemocyanin glycoconjugate (T-KLH). An additional IgM mAb (145.22) was produced in mice immunized with erythrocytes isolated from a patient with Tn syndrome. Three IgM and six IgG1 mAbs reactive with T-HSA did not react with natural T antigen present on desialyated glycophorin. All three IgM and several IgG1 mAbs, however, did react with LS-174T, a mucinous colon carcinoma cell line, 647V, a human bladder carcinoma cell line, and TA3Ha, a murine mammary carcinoma cell line as well as fresh frozen colon carcinomas. MAb 145.22 reacted with both natural and synthetic sources of sTn and Tn, as well as with LS-174T cells and mucin deposits in 10/11 colon carcinomas on fresh-frozen sections. MAb B72.3 reacted strongly with ovine submaxillary mucin (OSM) and sTn-HSA, while mAb CC49, a second-generation mAb to TAG-72 carcinoma mucin, reacted strongly with OSM, less strongly with desialyated OSM, and only weakly with sTn-HSA, suggesting that the epitope specificity for mAb CC49 is distinct from that of B72.3. |
| Databáze: | MEDLINE |
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