Exon mapping by fiber-FISH or LR-PCR.

Autor: Florijn RJ; Department of Cytochemistry and Cytometry, State University Leiden, Wassenaarseweg 72, Leiden, 2333 AL, The Netherlands., van de Rijke FM, Vrolijk H, Blonden LA, Hofker MH, den Dunnen JT, Tanke HJ, van Ommen GJ, Raap AK
Jazyk: angličtina
Zdroj: Genomics [Genomics] 1996 Dec 15; Vol. 38 (3), pp. 277-82.
DOI: 10.1006/geno.1996.0629
Abstrakt: In this study we systematically assessed the sensitivity limits of fiber-FISH in model experiments. Exonic fragments and cDNAs with exon sizes of >/=200 bp could be mapped on their cognate cosmid. This positional fiber-FISH mapping was validated by long-range PCR. It is expected that these two independent mapping approaches will help to refine current available gene maps and show their applicability in fine mapping of sequence-tagged sites or expressed sequence tags. Also, they will be useful in resolving gene structures by mapping exon and intron locations.
Databáze: MEDLINE