TGF-beta 1 forms functionally normal bone in a segmental sheep tibial diaphyseal defect.

Autor:	Moxham JP; Division of Otolaryngology-Head and Neck Surgery, University of British Columbia, Vancouver Hospital and Health Sciences Centre., Kibblewhite DJ, Dvorak M, Perey B, Tencer AF, Bruce AG, Strong DM
Jazyk:	angličtina
Zdroj:	The Journal of otolaryngology [J Otolaryngol] 1996 Dec; Vol. 25 (6), pp. 388-92.
Abstrakt:	Objective: Transforming growth factor-beta 1 (TGF-beta 1) is a polyfunctional regulatory cytokine that has been shown to have roles in extracellular matrix interactions, soft tissue healing, and osteogenesis. This study was undertaken to determine the efficacy of TGF-beta 1 in the formation of functionally normal bone in tibial-diaphyseal defects. Method: Seven hundred fifty micrograms of recombinant human TGF-beta 1 was added to a guanidine-extracted demineralised bone matrix (Gu-DBM) carrier and the implants were used to fill a 2.5 cm tibial diaphyseal defect in skeletally mature female sheep. The defects were allowed to heal over a 12-week period. After sacrifice, they were analyzed using four-point bending mechanical testing. Results: Implants with TGF-beta 1 showed complete bony bridging of the defect and stress-strain curves similar to the normal contralateral limb, while implants with the carrier alone failed to bridge the gap. Conclusions: These results demonstrate the ability of TGF-beta 1 to induce new bone which has structural and functional characteristics similar to normal bone.
Databáze:	MEDLINE
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