[Evaluation of methods of sperm preparation for human in vitro fertilization].
| Autor: | Mawissa D; Unité de Fertilité, Hôpital Universitaire Erasme (ULB), Bruxelles, Belgique., Legreve A, Ragon Y, Birmane J, Englert Y |
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| Jazyk: | francouzština |
| Zdroj: | Journal de gynecologie, obstetrique et biologie de la reproduction [J Gynecol Obstet Biol Reprod (Paris)] 1996; Vol. 25 (6), pp. 577-81. |
| Abstrakt: | Objectives: Compare results of sperm prepared with centrifiguation-migration, percoll discontinuous gradient (50, 70, 90%) and migration-sedimentation. Methods: An aliquot of sperm collected from 125 men referred for infertility was prepared according to the three methods. The spermogram after preparation and extration rates were compared using the Student's t test for paired variables and for three subpopulations identified by increasing spermatozoid concentrations. Results: Sperm count were significantly higher after percoll. Mobility and morphology were improved after centrifugation-migration excepting for the samples with the lowest counts. The extration rate was better after percoll and rose for the low-count samples. Conclusion: Percoll gradient is the most effective method, particularly for altered sperm. The number of mobile and normal forms can be improved with the centrifugation-migration technique. |
| Databáze: | MEDLINE |
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