HTLV Tax gene expression in patients with lymphoproliferative disorders.
| Autor: | Cardoso EA; Laboratório de Biologia Molecular (CIPM), Instituto Portugués de Oncologia, Lisboa, Portugal., Miranda N, Gameiro P, Frade MJ, Figueiredo M, Parreira A |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Journal of clinical pathology [J Clin Pathol] 1996 Nov; Vol. 49 (11), pp. 938-41. |
| DOI: | 10.1136/jcp.49.11.938 |
| Abstrakt: | Aims: To study the expression of the human T lymphotropic virus (HTLV) Tax gene in peripheral blood mononuclear cells. Methods: Blood was collected from 72 patients with lymphoproliferative disorders. Serum from all patients was assayed for antibodies directed against HTLV-I structural proteins by ELISA and western blotting. RNA was purified from fresh blood cells and amplified by reverse transcription polymerase chain reaction (RT-PCR). After Southern blotting, the PCR products were hybridised with a 32P end-labelled probe specific for the Tax gene. Results: All samples were seronegative. A specific band for the Tax gene was found in five samples. Each of the patients positive for Tax gene expression had a different type of lymphoproliferative disorder. Conclusions: Infection by HTLV-I cannot be assessed solely by immunological assays, particularly when only disrupted virions are used. Sensitive molecular biology assays are essential for detecting viral gene expression in fresh blood cells. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|