| Autor: |
Stewart AF; Department of Medicine, University of Pittsburgh, Pennsylvania 15213, USA. als6+@pitt.edu, Richard CW 3rd, Suzow J, Stephan D, Weremowicz S, Morton CC, Adra CN |
| Jazyk: |
angličtina |
| Zdroj: |
Genomics [Genomics] 1996 Oct 01; Vol. 37 (1), pp. 68-76. |
| DOI: |
10.1006/geno.1996.0522 |
| Abstrakt: |
Transcriptional Enhancer Factor-1 (TEF-1) is a transcription factor required for cardiac muscle gene activation. Since ablation of TEF-1 does not abolish cardiac gene expression, we sought to identify a human gene related to TEF-1 (RTEF-1) that might also participate in cardiac gene regulation. A human heart cDNA library was screened to obtain a full-length RTEF-1 cDNA. Fluorescence in situ hybridization assigned the RTEF-1 gene to chromosome 12p13.2-p13.3. In contrast, PCR screening of human/rodent cell hybrid panels identified TEF-1 on chromosome 11p15.2, between D11S1315 and D11S1334, extending a region of known synteny between human chromosomes 11 and 12 and arguing for an ancient divergence between these two closely related genes. Northern blot analysis revealed a striking similarity in the tissue distribution of RTEF-1 and TEF-1 mRNAs; skeletal muscle showed the highest abundance of both mRNAs, with lower levels detected in pancreas, placenta, and heart. Phylogenetic analysis of all known TEF-1-related proteins identified human RTEF-1 as one of four vertebrate members of this multigene family and further suggests that these genes diverged in the earliest metazoan ancestors. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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