| Autor: |
Kruman II; Department of Microbiology and Immunology, University of Kentucky, Lexington 40536-0230, USA., Ramiya V, Bondada S |
| Jazyk: |
angličtina |
| Zdroj: |
Cellular immunology [Cell Immunol] 1996 Nov 01; Vol. 173 (2), pp. 236-45. |
| DOI: |
10.1006/cimm.1996.0273 |
| Abstrakt: |
Using a monoclonal antibody to CD4 we have shown that occupation of CD4 on T cells induces a strong dose dependent inhibition of in vitro IgM plaque forming cell (PFC) response of spleen cells to the T dependent antigen (Ag), sheep red blood cells (SRBC), in Mishell-Dutton cultures. This inhibitory effect is not due simply to nonspecific perturbation or Fc binding, since F(ab) fragments of anti-CD4 are as potent as the intact antibodies, whereas antibodies to class I molecules or T cell CD5 have no effect. The anti-CD4 antibody appears to block contact dependent interaction between T and B cells and this inhibitory effect cannot be overcome by cytokines. Anti-CD4 did not inhibit the PFC response to the T independent antigen, trinitrophenylated lipopolysaccharide. The anti-CD4 antibody prevented the interaction of preactivated fixed SRBC specific T helper cells with B cells, suggesting that CD4 had a role in contact mediated interactions between T cells and B cells. Surprisingly, antibodies to CD40L failed to inhibit the SRBC specific PFC response. Thus CD4 appears to be an important molecule required for cognate interactions between T and B cells that are needed to generate an Ag specific PFC response. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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