| Autor: |
de Zwart LL; Leiden/Amsterdam Center for Drug Research, Free University, Department of Pharmacochemistry, The Netherlands., Hermanns RC, Meerman JH, Commandeur JN, Vermeulen NP |
| Jazyk: |
angličtina |
| Zdroj: |
Xenobiotica; the fate of foreign compounds in biological systems [Xenobiotica] 1996 Oct; Vol. 26 (10), pp. 1087-100. |
| DOI: |
10.3109/00498259609167424 |
| Abstrakt: |
1. 4-Hydroxy-2,3-nonenal (HNE) is an end product of lipid peroxidation (LPO) and a well known cytotoxic aldehyde that exhibits a variety of biological effects. In this study the in vivo disposition and covalent binding of i.p. administered [2-3H]HNE was examined in the rat. 2. It was found that several metabolites of [2-3H]HNE are excreted in urine among which at least four mercapturic acids. 1,4-Dihydroxynonane mercapturic acid (DHN-MA) appeared to be the most abundant mercapturic acid excreted in urine (3.5% of the dose) and the excretion of the other three mercapturic acids amounted to 2% of the dose. 3. Within 48 h following i.p. administration of 5 or 25 mumol/kg bodyweight [2-3H]HNE (specific activity 4 microCi/mumol) about 25% of the radioactivity was excreted in urine, whereas 18% of the radioactivity appeared in the faeces. 4. After 48 h, 7% of the radioactivity was still present in the liver and 0.2% in other organs, but this radioactivity appeared to not to be covalently bound to cellular macromolecules. It was found that only 0.13% of the radioactivity was covalently bound in the liver and even less in other organs. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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