Comparison of three urease tests for detection of Helicobacter pylori in gastric biopsy specimens.

Autor:	Boyanova L; Department of Microbiology, Medical University, Sofia, Bulgaria., Stancheva I, Todorov D, Kumanova R, Petrov S, Vladimirov B, Pehlivanov N, Mitova R, Chakarski I, Churchev I
Jazyk:	angličtina
Zdroj:	European journal of gastroenterology & hepatology [Eur J Gastroenterol Hepatol] 1996 Sep; Vol. 8 (9), pp. 911-4.
Abstrakt:	Objective: To propose two modified urease tests for the detection of Helicobacter pylori in gastric biopsy specimens. Patients and Methods: The presence of H. pylori infection was determined in 237 patients undergoing upper gastrointestinal endoscopy. Three media were used for the urease tests: Christensen's 2% urea broth and two urea agar media, modified by increasing the concentration of urea (to 4% and 10%) and phenol red and omitting the nutrients. Results: The modified tests had good sensitivity (> 78%), specificity (96%) and accuracy (> or = 86%) at 2 h using small amounts (15%) of biopsy homogenates. They were statistically more sensitive and accurate than Christensen's broth. Conclusion: Both the modified 4% and 10% urea agar tests are simple, sensitive and specific and can be performed with small amounts of sample.
Databáze:	MEDLINE
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