| Autor: |
Jestice HK; Department of Clinical Haematology, Addenbrooke's NHS Trust, Cambridge, UK., Boraks P, Scott MA, Bloxham D, Price J, Marcus RE |
| Jazyk: |
angličtina |
| Zdroj: |
Bone marrow transplantation [Bone Marrow Transplant] 1996 Sep; Vol. 18 (3), pp. 597-601. |
| Abstrakt: |
The efficiency of leucapheresis using the Cobe Spectra depends on the observation of the operator to monitor the haematocrit (Hct) in the collection line at the recommended Hct of 1%. Cells were harvested by processing 3 x total blood volume (TBV) over 3-4 h. During the harvest the manual Hct and the colony-forming units granulocyte-macrophage (CFU-GM) concentrations were analysed in the collection line in the observed Hct range of 1-7.5%. There was a correlation between the manual and observed Hct (r = 0.97). The CFU-GM concentration was normalised to allow comparison between patients. The increase was statistically significant between 1-2% (P = 0.04) and 1-3% Hct (P = 0.05). The increase in CFU-GM concentration remained at the termination of harvest, indicating that not all available CFU-GM were harvested. The optimum concentration of progenitor cells was found to be at 3% Hct. We postulate that this may permit the collection of sufficient cells on one occasion to allow PBPC autografting in the majority of patients who respond to mobilisation. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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