| Autor: |
Lynch KM; SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania, USA., Sellers TS, Gossett KA |
| Jazyk: |
angličtina |
| Zdroj: |
European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies [Eur J Clin Chem Clin Biochem] 1996 Jul; Vol. 34 (7), pp. 569-71. |
| Abstrakt: |
Methods for quantitating urinary protein differ in their ranges of linearity, technical ease of performance, and applicability to automated analyzers. The Coomassie Brilliant Blue method is widely used but has limited linearity and its tendency to stain glassware has limited its application to automated analyzers. We evaluated a pyrogallol red-molybdate protein dye-binding method (Biotrol USA, Inc.) on a Hitachi 705 analyzer for the quantitation of urinary protein in rats. This method showed a wide range of linearity (up to 2.6 g/l) and good precision. Within-run CVs of 6.6% and 1.3% and between-day CVs of 10.9% and 1.1% were observed at mean protein concentrations of 0.16 g/l and 1.96 g/l, respectively. In addition, rat urine protein results from this method correlated well (r2 = 0.998, n = 40) with a Coomassie Brilliant Blue method (QuanTtest Blue, Quantimetrix Corporation). No significant or unexpected interferences were encountered with this method. We conclude that the automated pyrogallol red-molybdate method is an acceptable and practical alternative to the Coomassie Brilliant Blue method for the quantitation of urine protein in rats. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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