| Autor: |
van Binsbergen J; Organon Teknika, Boxtel, The Netherlands., de Rijk D, Peels H, Dries C, Scherders J, Koolen M, Zekeng L, Gürtler LG |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of virological methods [J Virol Methods] 1996 Jul; Vol. 60 (2), pp. 131-7. |
| DOI: |
10.1016/0166-0934(96)02050-2 |
| Abstrakt: |
Although the HIV-1 group O virus found in two persons of Cameroonian origin has been described in 1990 (De Leys et al., 1990), sera from group O infected individuals became available only recently. Several studies showed that some of the anti-HIV-1/HIV-2 screening tests failed to detect all of these samples (Loussert-Ajaka et al., 1994; Simon et al., 1994; Schable et al., 1994; Gürtler et al., 1995). In the current version of an anti-HIV-1/anti-HIV-2 screening assay, namely the Vironostika HIV Uni-Form II, an HIV-O specific peptide was introduced in order to improve HIV-1 group O reactivity. The peptide was derived from the immunodominant region of HIV-1 group O gp41 strain ANT70. All 30 anti-HIV-1 group O sera were detected by the so-called plus O assay, while 29 samples of this panel were positive the current assay. The sensitivity of the plus O assay for anti-HIV-1 and anti-HIV-2 positive samples is identical to that of the reference test without HIV-1 group O peptide. The clinical specificity of the HIV Uni-Form II plus O assay is improved to > or = 99.92% by an adjustment of the coat concentration of HIV-1 p24 (to avoid false positive p24 only reactions) without affecting sensitivity of the assay. The specific reaction of an HIV-1 group O specific rabbit serum for quality control purposes is presented. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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