| Autor: |
Shliapnikova OV; Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia., Efimenko AN, Bulatova TI, Liaĭman ME, Vertiev YV |
| Jazyk: |
ruština |
| Zdroj: |
Zhurnal mikrobiologii, epidemiologii i immunobiologii [Zh Mikrobiol Epidemiol Immunobiol] 1996 Jan-Feb (1), pp. 23-6. |
| Abstrakt: |
Polymerase chain reaction (PCR) for the identification of C.botulinum of type A was developed. As primers, oligonucleotides corresponding to sequences 913 -- 932 and 1852 -- 1871 of the gene of type A botulinic neurotoxin were used. The study revealed that under optimum conditions the positive result of the reaction was registered only when the DNA of C.botulinum strains of type A (11 strains) was used, but not that of C.botulinum strains of other types (11 strains of type B, 5 strains of type C, 2 strains of type D, 6 strains of type E and 1 strain of type G). High sensitivity, specificity and rapidity of PCR open good prospects for its practical use. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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