| Autor: |
Ghiorse WC; Section of Microbiology, Division of Biological Sciences, Cornell University, Ithaca, New York 14853, USA., Miller DN, Sandoli RL, Siering PL |
| Jazyk: |
angličtina |
| Zdroj: |
Microscopy research and technique [Microsc Res Tech] 1996 Jan 01; Vol. 33 (1), pp. 73-86. |
| DOI: |
10.1002/(SICI)1097-0029(199601)33:1<73::AID-JEMT7>3.0.CO;2-1 |
| Abstrakt: |
Laser scanning microscopy was used 1) to analyze Mn oxide-encrusted biofilms and particles in marine Mn-oxidizing enrichment cultures, 2) to optimize fluorescence in situ hybridization protocols of 16S ribosomal RNA-targeted oligonucleotide signature probes for single bacterial cell identification in particles from a wetland, and 3) to develop a combined immunofluorescence-microautoradiography procedure for analysis of the distribution of 14C-labeled organic compounds and 14C-mineralizing bacteria in groundwater seep sediments. The results demonstrated the wide applicability and benefits of using laser scanning microscopy for analysis of complex microbial assemblages. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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