| Autor: |
Kaye B; Department of Drug Metabolism, Pfizer Central Research, Sandwich, Kent, U.K., Herron WJ, Macrae PV, Robinson S, Stopher DA, Venn RF, Wild W |
| Jazyk: |
angličtina |
| Zdroj: |
Analytical chemistry [Anal Chem] 1996 May 01; Vol. 68 (9), pp. 1658-60. |
| DOI: |
10.1021/ac9507552 |
| Abstrakt: |
A novel method has been developed for the rapid solid phase extraction of drugs and metabolites from biological fluids, prior to further analysis. The newly designed, 96-tube micropreparation block facilitates high throughput by enabling the extraction of 96 samples simultaneously. The system is described, linked to HPLC/APCI-MS/MS, for the determination of darifenacin in human plasma. The resulting procedure, using deuterated darifenacin as internal standard, is validated over the concentration range 25-2000 pg/mliter; accuracy (0.6-4.6%) and precision (3.6-18.8%) are considered acceptable and overall recovery was determined to be approximately 50%. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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