| Autor: |
Buc HA; Laboratoire de Biochimie Métabolique et Pharmacologique, INSERM U75, Paris, France., Moncion A, Pérignon JL |
| Jazyk: |
angličtina |
| Zdroj: |
International journal of immunopharmacology [Int J Immunopharmacol] 1996 Feb; Vol. 18 (2), pp. 135-9. |
| DOI: |
10.1016/0192-0561(95)00116-6 |
| Abstrakt: |
Cross-talk between signalling pathways appears to play an important role in T-lymphocyte activation. In the present work, we have studied the effects of different inhibitors of protein tyrosine kinases or protein serine/ threonine kinases on the agonist-induced cAMP accumulation in the human T-lymphoblast cell line Jurkat. Staurosporine, a potent but nonspecific inhibitor of protein kinases, produced a ten-fold enhancement of the response to PGE2. No significant effect was obtained with two specific protein kinase C inhibitors (GF 109203X and H7), whereas herbimycin A, a specific protein tyrosine kinase inhibitor, markedly enhanced the PGE2-induced cAMP accumulation: its effect was approximately 60% that of staurosporine. It was confirmed that both staurosporine and herbimycin A inhibited by more than 90% the release of IP3 induced by ligation of the T-cell receptor, a known protein tyrosine kinase-dependent mechanism. To our knowledge, this study provides the first indication of a protein tyrosine kinase-mediated inhibition of agonist-induced cAMP accumulation. The possible targets of this inhibition are discussed. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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